Experimental in vitro phenotype reprogramming of two subsets of neutrophilic granulocytes in children with acute destructive pneumonia by means of a synthetic hexapeptide

Effector dysfunctions of neutrophil granulocytes are often associated with the occurrence dysregulatory processes in antibacterial immune defense. Acute destructive pneumonia is a severe purulent-inflammatory disease discordant functions effector mechanisms and emergence negatively transformed cell subsets. Therefore, search for new experimental approaches aimed at re-orientation altered phenotype distinct subsets neutrophilic children acute by means various immunotropic substances quite relevant. The aim study was to evaluate modulating effects synthetic hexapeptide (Arginyl-alpha-Aspartyl-Lysyl-Valyl-Tyrosyl-Arginine) on contents 2 functionally significant major (CD16+CD64-CD32+CD11b+) minor (CD16+CD64+CD32+CD11b+) subpopulations neutrophils closed vitro system sampled atypical pneumonia. We have examined twenty peripheral blood samples from 10 pneumonia, 40 20 healthy 2-4 years old. Immunophenotyping classified performed basis expression density membrane receptors, according MFI criteria. Phenotypic features granulocyte were evaluated before after incubation Hexapeptide (10-6 g/L; 37 C, 60 min). In compared conditionally children, following variants negative transformation established: decrease ratios subset, i.e., 98.0 (96.9-98.7) % o 55.8 (35.3-74.8) %, decreased CD16 CD11b MFI, significantly increase ratio subset: 1.3 (0.4-1.6) 52.6 (41.8-54.9) increased receptor, CD64 expression. Immunomodulatory upon been demonstrated showing positive remodeling both absence quantitative changes. Thus, treatment hexapeptide, we found activation receptors CD16, their subset levels typical children. At same time, did not affect studied except subset. obtained data can be used future develop targeted immunotherapy correcting